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OBJECTIVE: To study the effect of verapamil on the expression of transforming growth factor-β1 (TGFβ1), TGF-β2 and extracellular marix(ECM) synthesis in bovine trabecular meshwork ceUs(BTMC) cells(BTMQ. METHODS: The effect of verapamil equal to or less than 0.01 mg/mL on the expression of TGF-β1, TGF-β2 in BTMC was detected by immunohistochemistry with computer-aided color image analysis and semi-quantitative RT-PCR analyses, respectively. RESULTS: 0.001, 0.005 and 0.01 mg/mL of verapamil could inhibit the expression of TGF-β1, TGF-β2 in BTMC at the level of protein and mRNA in a concentration-dependent manner (P < 0.05), and 0.01 mg/mL verapamil could inhibit the protein synthesis of TGF-β 1 completely. The same concentration of verapamil inhibited the expressions of TGF-β1 more than TGF-β2. CONCLUSION: The possible mechanism for inhibiting the ECM synthesis by verapamil may be related to decreasing TGF-β expression, and the inhibition of TGF-β1 expression may play a principle role. This result further provides an experimental basis for the prospect of pathogenetic treatment of primary open-angle glaucoma(POAG) by verapamil. LA: Chinese
F. Chen, Department of Ophthalmology, Union Hospital, Huazhong Univ. of Sci. and Technol., Wuhan 430022
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)