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Abstract #18478 Published in IGR 3-3
The changes of vitreous pH values in an acute glaucoma rabbit model
Lu DW; Chang CJ; Wu JNJournal of Ocular Pharmacology and Therapeutics 2001; 17: 343-350
The changes in vitreous pH values under acute glaucoma status have never been reported. In this study, the authors measured the changes in vitreous pH values in an acute glaucoma rabbit model. Under ketamine and xylazine anesthesia, the anterior chamber of the right eye of New Zealand white rabbits was cannulated and connected to a polygraph for continuous intraocular pressure (IOP) monitoring, and to a mini-pump for persistent injection of viscoelastic substance, Healon GV. The measurement of vitreous pH was done by inserting a pH probe via a third opening through the sclera into the vitreous. Different IOP levels were maintained by varied amounts of Healon GV injections into the anterior chamber. The changes of vitreous pH values following the intracameral injections were recorded and evaluated. The changes of vitreous pH values were minimal when IOPs were maintained at lower than 35 mmHg. Vitreous pH values decreased significantly from 7.32 to less than 7.03, when the IOPs were kept at 70 mmHg or higher. However, the decrease of pH values was reversible if IOP was held at 70 mmHg for less than ten minutes and then immediately reduced to normal levels. In addition, with MTT viability assay, it was noted that the decrease in vitreous pH was associated with a higher percentage of retinal cell death.
Dr D.W. Lu, Department of Ophthalmology, Tri-Service General Hospital, Cheng-Kong Road, Taipei 114, Taiwan. p310849@ms23.hinet.net
Classification:
2.16 Chiasma and retrochiasmal central nervous system (Part of: 2 Anatomical structures in glaucoma)