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Abstract #20376 Published in IGR 10-1
The study of retinal ganglion cell apoptosis induced by different intensities of microwave irradiation
Zhou XR; Yuan HP; Qu W; Ma CY; Li HY; Wang YOphthalmologica 2008; 222: 6-10
PURPOSE: To observe the influence on the retinal ganglion cells induced by 2,450-MHz microwave. The radiant intensities were 10, 30 and 60 mW x cm-2. METHODS: Retinal ganglion cells were cultured in vitro and divided into 4 groups, of which 3 were exposed to different intensities of microwaves and 1 was the control group. The morphological variation of cells was observed by invert microscope. The survival rate was assessed by trypan blue. Annexin V-PI 2-color flow cytometry was used to detect the percentage of earlier apoptosis cells after radiation. RESULTS: The changes in the morphology of all cells were observed after radiation. The cell survival rates were reduced and the earlier apoptosis increased with growing microwave intensity. CONCLUSIONS: The 2,450-MHz microwave will cause damage to retinal ganglion cells in a dose-dependent manner.
Dr. X.R. Zhou, Department of Ophthalmology, Harbin Medical University, Second Affiliated Hospital, Harbin, China
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.6 Cellular biology (Part of: 3 Laboratory methods)
