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Abstract #20875 Published in IGR 10-2
Increased plasma and optic nerve levels of IL-6, TNF-α, and MIP-2 following induction of ischemic optic neuropathy in mice
Avraham BC; Dotan G; Hasanreisoglu M; Kramer M; Monselise Y; Cohen Y; Weinberger D; Goldenberg-Cohen NCurrent Eye Research 2008; 33: 395-401
PURPOSE: To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION). METHODS: AION was induced in C57/BL6 mice and levels of IL-6, TNF-α, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals. RESULTS: Plasma: IL-6 levels were elevated immediately after Raion induction and decreased gradually thereafter. TNF-α showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-α, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3. CONCLUSIONS: Proinflammatory cytokines may play a role in the pathogenesis of Raion.
Dr. B.C. Avraham, The Krieger Eye Research Laboratory, Felsenstein Medical Research Center, Petach Tikva, Israel
Classification:
10 Differential diagnosis e.g. anterior and posterior ischemic optic neuropathy
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
3.10 Immunobiology (Part of: 3 Laboratory methods)