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OBJECTIVE: The rat is an ideal animal as a model of general, corneal and retinal wound healing, but rabbit has been used as a standard animal model for wound healing and scarring process following glaucoma filtering surgery. Difference from rat,mouse and non-human primate models, the genetic sequences of rabbits for this type of work has not been well defined. The purpose of this investigation was to establish a modified operative model of glaucoma filtering surgery. METHODS: Glaucoma filtering surgery was performed on the right eyes of 10 Sprague-Dawley rats by introducing a 29-gauge silicone cannula through a penetrating scleral tunnel under a limbal-based conjunctival flap. Identical glaucoma filtering surgery was performed on other 3 rats for the histologic evaluation at day 2, 5, 11 following surgery. RESULTS: Following the cannula of glaucoma filtering surgery, well-elevated filtering blebs formed and gradually failed over the course of 8 to 13 days. The sub-conjunctional bleb was elevated and avascular in 24 hours after surgery and gradually flatten and vascularized 5 days later. The inflammatory cells was gradually increased around the bleb in the 1-5 days of postoperation and decreased 11 days later and the connective tissue was gradually density with lapse of postoperation time. The intraocular pressure was lowest in the day 1 after operation and returned to the baseline level in 5 and 6 days postoperatively. No any complication occurred in model rat after operation. CONCLUSION: Rat model of cannulating filtering surgery offers a long lasting and predictable method for the studies of wound healing following glaucoma filtering surgery and induced changes at the gene level. LA: Chinese
Dr. H. Lu, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
12.8 Filtering surgery (Part of: 12 Surgical treatment)