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Abstract #26278 Published in IGR 12-2
Angiotensin receptors in the eyes of arterial hypertensive rats
Vaajanen A; Lakkisto P; Virtanen I; Kankuri E; Oksala O; Vapaatalo H; TikkanenActa Ophthalmologica 2010; 88: 431-438
Purpose: The aim of the present study was to determine whether the eye tissues of arterial hypertensive rats evince expression of angiotensin receptors (AT1 and AT2) as well as the novel Mas receptor, whose endogenous ligand is vasorelaxing Angiotensin (1–7) [Ang (1–7)]. Methods: Enucleated eyes from spontaneously hypertensive rats (SHR) and double transgenic rats harbouring human renin and angiotensinogen genes (dTGR) and their normotensive controls were used. Half of the rats were pretreated orally with an Angiotensin II (Ang II) type 1 receptor blocker (ARB). The eyes were snap-frozen in isopentane at ?40° and stored at ?70° for subsequent reverse transcriptase polymerase chain reaction (RT-PCR) analysis or in vitro autoradiography. Results: The mRNA expression of AT1a and AT 2 as well as the novel Mas receptor was detected in all rat groups, being markedly higher in the retina than in the ciliary body. dTGR had significantly more receptors than SHR, but no direct relation to blood pressure level was seen. According to the autoradiography, treatment with ARB blocked a part of AT1 receptors but had no clear effect on AT2 receptors. Conclusion: The novel Mas receptor was found by RT-PCR in eye tissue for the first time. Its specific ligand, Ang (1–7), may be involved in the regulation of intraocular pressure – as recently demonstrated by us – and in the pathogenesis of retinal diseases as a counter-regulatory component for the vascular and proliferative actions of Ang II. The results suggest that the density of AT1 receptors in the eye is independent of the blood pressure level of the animal.
Department of Pharmacology, Institute of Biomedicine, University of Helsinki, Finland
Classification:
3.8 Pharmacology (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
