advertisement
Abstract #45944 Published in IGR 13-2
Effects of huoxuehuayu prescription on retina with acute intraocular hypertension
Zhang C; Wang D-B; Fang J; Shang W-WChinese Ophthalmic Research 2010; 28: 1129-1133
Background: Research showed that traditional Chinese medicine can protect effectively retinal ganglion cells (RGCs) against injury induced by intraocular hypertension. However, the mechanism is not very clear up to now. Objective: This study was designed to evaluate the protective role of huoxuehuayu prescription on rat retina injured by acute intraocular hypertension and its mechanism. Methods: One hundred clean Wistar rats were randomly divided into normal group, control group (10 rats), punctural group (10 rats), model group (40 rats) and huoxuehuayu prescription group (40 rats). Huoxuehuayu prescription agent was prepared by the mixture of several kinds Chinese herbs. Ocular hypertension models were established by perfusion of normal saline solution into the anterior chamber to elevate the intraocular pressure (IOP) to 66 mmHg in 40 rats, and 2 mL huoxuehuayu prescription solution was administered intragastrally 2 times per day from modeling through death in 20 models. The rats in different groups were sacrificed respectively on 1, 3, 7, 14 days after compression. The retina was histologically examined by H&E staining under the light microscope. The expression of brain-derived neurotrophic factor (BDNF) protein and mRNA were detected respectively by immunofluorescence and real-time reverse transcription polymerase chain reaction (real-time PCR) analysis. Results: In the model group, histological examination showed that in the first day after elevation of IOP, edema of retina, decrease of retinal ganglion cells (RGCs), vacuolar degeneration of cells were more obvious, and in the second day, the histological changes of retina was mild. However, in the 7th to 14th day, the attenuation and atrophy of retina thickness, disorder of retinal structure, apoptosis of RGCs were found. However, in hypertension group, retinal damage was slight at various time points in comparison with model group. The expression of BDNF protein was upregulated and peaked on 1 day after intraocular hypertension and descended later in 3, 14 days in model group and huoxuehuayu group. The expression level in huoxuehuayu prescription group was significantly higher than that in model group at the same time points(P < 0.05). The expression of BDNF mRNA showed the same tendency to the results of the BDNF protein expression, showing evidently increase in huoxuehuayu prescription group compared with model group at various time points (P < 0.05). Conclusion: Huoxuehuayu prescription can protect the rat retina against injure induced by acute intraocular hypertension by promoting the expression of endogenous BDNF in retina.
C. Zhang. Department of Ophthalmology, Qingdao University Medical College, Qingdao 266003, China.
Classification:
15 Miscellaneous
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
