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Abstract #47120 Published in IGR 13-3
Development and Expression of Amyloid-(beta) Peptide 42 in Retinal Ganglion Cells in Rats
Wang J; Zhu C; Xu Y; Liu B; Wang M; Wu KAnatomical RecordProceedings of the National Academy of Sciences of the United States of America 2011; 294: 1401-1405
The previous studies have shown that Amyloid-(beta) peptide (A(beta)) was mainly found in neurons of neurodegenerative diseases, such as Alzheimer's disease (AD) and glaucoma and little is known about its expression in normal nerve cells. The aim of the present study was to investigate the expression of amyloid-(beta) peptide 42 (A(beta)-42) in retinal ganglion cells of the postnatal rats. Rats were divided into seven experimental groups: 3, 6, 13, 15, 25, 60, and 90 days postnatal groups. Rats from 15 and 25 days postnatal groups were further divided into light-exposure and non light-exposure group. Cryosections or flat-mounted retinas of rat eyes were used for testing A(beta)-42 by immunocytochemistry staining. A(beta)-42 expression was not observed in rats within 13 days after birth, but was easily detectable in all groups of rats over 15 days after birth. In addition, the expression of A(beta)-42 in retina was increasing as the rats got older, reached to highest level in 60 days after birth. Furthermore, the expression of A(beta)-42 was not detected in rats kept under dark indicating that light is required for the expression of A(beta)-42 in retina. This is the first report showing that normal retinal ganglion cells express A(beta)-42, and that the expression of A(beta)-42 in retinal ganglion cells requires the exposure to light. These data suggest that A(beta)-42 may play a important role in vision development.
K. Wu. Department of Anatomy, Medical College, Soochow University, Suzhou, China.
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
