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Abstract #6321 Published in IGR 3-2
Effect of H-7 on cultured human trabecular meshwork cells
Liu X; Cai S; Glasser A; Volberg T; Polansky JR; Fauss DJ; Brandt CR; Geiger B; Kaufman PLMolecular Vision 2001; 7: 145-153
PURPOSE: To determine the effect of the serine-threonine kinase inhibitor H-7, which blocks actomyosin contractility and increases outflow facility in live monkeys, on morphology, cytoskeleton, and cellular adhesions of human trabecular meshwork (HTM) cells in culture. METHODS: Cultured HTM cells were videographically recorded and evaluated before and after exposure to H-7 at different concentrations. The subcellular distribution of the actin-based cytoskeleton and associated anchor proteins including vinculin, paxillin, and beta-catenin, as well as phosphotyrosine-containing proteins were evaluated by fluorescence immunocytochemistry and digital fluorescence microscopy. RESULTS: H-7 induced pronounced but reversible HTM cell thickening toward the cell center and deterioration of the actin cytoskeletal network. Cell-extracellular matrix (ECM) and cell-cell adhesions were also affected, but the beta-catenin-rich, vinculin-containing adherens junctions were clearly more resistant than focal contacts. Phosphotyrosine labelling in focal contacts was highly sensitive to H-7. CONCLUSIONS: H-7 induces alterations in cell shape, actin cytoskeleton, and associated focal adhesions in cultured HTM cells, which may be responsible for the effects of H-7 on outflow facility in live monkey eyes.
Dr X. Liu, Department of Ophthalmology & Visual Sciences, University of Wisconsin, Madison, WI 53792, USA. xliu5@facstaff.wisc.edu
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
4 Tissue culture of ocular cells
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
