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Abstract #6328 Published in IGR 3-2
The quantification of endogenous steroids in bovine aqueous humour and vitreous humour using isotope dilution GC-NCI-MS
Iqbal Z; Midgely JM; Watson DGJournal of Pharmacological and Biomedical Analysis 2001; 24: 535-543
Pentafluorobenzyloxime-trimethylsilyl derivatives of androgens, progestogens and corticosteroids were prepared and used for the analysis of these steroids in bovine aqueous humor and vitreous humor by the GC-MS method. Appropriate deuterated isotopomers of the parent steroids were labelled with deuterium via simple synthetic procedure and used as internal standards. The concentration (ng ml-1, ± SEM) of these steroids in bovine aqueous humor and vitreous humor were found to be as follow: (1) aqueous humor (n = 17); hydrocortisone (n = 17; 2.40 ± 0.54), progesterone (n = 15; 0.06 ± 0.01), 4-androstene-3,17-dione (n = 8; 0.15 ± 0.07) and testosterone (n = 4; 0.14 ± 0.04); and (2) bovine vitreous humor (n = 19); hydrocortisone (n = 19; 1.78 ± 0.25), progesterone (n = 18; 0.09 ± 0.01), 4-androstene-3,17-dione (n = 19; 0.11 ± 0.02), 11-deoxycorticosterone (n = 12; 29.27 ± 6.42), 17α-hydroxyprogesterone (n = 6; 5.55 ± 3.12). The concentration of corticosterone, 11-deoxycorticosterone and 17α-hydroxyprogesterone and testosterone and corticosterone were below the limit of detection in aqueous humor and vitreous humor, respectively.
Dr Z. Iqbal, Department of Pharmaceutical Science, University of Strathclyde, Glasgow, UK. zafariqbal_735@yahoo.com
Classification:
2.6 Aqueous humor dynamics (Part of: 2 Anatomical structures in glaucoma)
5 Experimental glaucoma; animal models
