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Abstract #8869 Published in IGR 5-2
Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy mouse eyes
Grozdanic SD; Betts DM; Allbaugh RA; Sakaguchi DS; Kwon YH; Kardon RH; Sonea IMCurrent Eye Research 2003; 26: 371-378
PURPOSE: To characterize the pupil light reflex (PLR), electroretinographic (ERG) and tonometric parameters which might be of importance for the in vivo characterization of mouse models of chronic ocular hypertension. METHODS: C57/BL6 mice were used for experiments. The PLR was evaluated with a computerized pupillometer (n = 14), ERGs were recorded simultaneously from both eyes (n = 23) and IOP was measured with a modified Goldmann tonometer (n = 23). RESULTS: The analysis of the PLR parameters confirmed the consensual PLR did not have significantly different amplitude (p > 0.1) and latency time (p > 0.1) compared to the direct PLR. However, PLR velocity (p = 0.004) was significantly smaller in the consensual PLR. Electroretinography revealed a-wave amplitude of 168.3 ± 9.6 μV with latency of 27.5 ± 0.6 msec and b-wave 403 ± 28.8 μV with latency of 22.7 ± 0.6 msec. The flicker ERG recording revealed amplitudes of 20.6 ± 2.4 μV. Tonometry experiments revealed that modified Goldmann tonometer measurements correlated well with invasive manometry (r2 = 0.89). The mean IOP of the mouse was 15.3 ± 0.6 mmHg. CONCLUSIONS: Consensual PLR in mice is relatively slower than the direct PLR, but retains the same degree of constriction comparing to the direct PLR. A modified Goldmann tonometer seems to be a reliable non-invasive tool for IOP measurements in mice.
Dr. S. Grozdanic, College of Veterinary Medicine, Iowa State University, Department of Veterinary Clinical Sciences, Ames, IA, USA
Classification:
5 Experimental glaucoma; animal models
6.1 Intraocular pressure measurement; factors affecting IOP (Part of: 6 Clinical examination methods)
6.7 Electro-ophthalmodiagnosis (Part of: 6 Clinical examination methods)
