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Abstract #124942 Published in IGR 25-2
Mesenchymal stem cell-derived exosomes carry miR-125a-5p to improve diabetic keratopathy by regulating endoplasmic reticulum stress
Li W; He S; Lin C; Yang S; Zhang WTissue & cell 2025; 93: 102669
BACKGROUND: Diabetic keratopathy is a prevalent but sometimes ignored visual condition in diabetic patients, which significantly affects patients with diabetes mellitus (DM) in terms of their visual acuity. Exosomes regulate diabetes-related conditions like diabetic keratopathy (DK) by secreting their components into the body. OBJECTIVE: AIM: to investigate the effect and mechanism of mesenchymal stem cell (MSC)-derived exosome miR-125a-5p on DK. METHODS: Transmission electron microscopy, along with nanoparticle tracking analysis, was used to determine the morphology and size of exosomes. To evaluate cell viability, proliferation, and migration, Western blotting and RT-qPCR methods were used. CCK-8, cell cloning, and scratch assays were used to measure protein levels and mRNA expression. RESULTS: High glucose treatment of corneal epithelial cells weakened cell viability, proliferation and migration, and the level of miR-125a-5p was significantly reduced. It has been proposed that elevated levels of miR-125a-5p could enhance cell viability, proliferation, and migration, can inhibit endoplasmic reticulum stress induced by high glucose, which is the same as the effect of endoplasmic reticulum stress inhibitors. CONCLUSION: Mouse bone marrow MSC-derived exosome miR-125a-5p repairs corneal epithelial cell viability and proliferation as well as migration ability to improve DK by inhibiting high glucose-induced endoplasmic reticulum stress.
Ophthalmology Department, Nanxishan Hospital of Guangxi Zhuang Autonomous Region (The second Hospital of Guangxi Zhuang Autonomous Region), Guilin, Guangxi 541000, China; Ophthalmology Department, The Second Affiliated Hospital of Guangxi University of Science and Technology, Liuzhou, Guangxi 545005, China. Electronic address: liweina176@163.com.
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