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Abstract #15661 Published in IGR 2-3
Mechanism related to reduction of intraocular pressure by melanocortins in rabbits
Naveh N; Kaplan-Messas A; Marshall JBritish Journal of Ophthalmology 2000; 84: 1411-1414
AIM: To investigate whether the ocular hypotensive effect of alpha melanocyte stimulating hormone (MSH) is related to eicosanoids or cyclic AMP (cAMP). METHODS: Intraocular pressure (IOP) readings were taken at a similar time on the day before and after a single dose of topical MSH. Changes in the levels of prostaglandin E(2) (PGE(2)) and prostacyclin in incubated iris ciliary body (ICB) explants were measured by specific radioimmunoassay (RIA). Incubated ICB explants were exposed to MSH or adrenaline (epinephrine) for a week. In addition, cAMP levels in the medium were determined following short term incubation using RIA. RESULTS: A significant dose related reduction in IOP was noted with topical MSH (mean (SD) maximal effect 4.5 (0.1) mmHg (21%); p < 0.001 versus appropriate baseline) which persisted up to six hours (p = 0.05). MSH-treated ICB explants showed a 1.5-fold increase in PGE(2) and prostacyclin levels (p < 0.001 for each parameter), while cAMP levels were increased twofold (p < 0.001). CONCLUSIONS: A single application of MSH caused a sustained dose related ocular hypotensive effect with no side effects. An increase in eicosanoid and cAMP levels following ICB exposure to MSH indicated their involvement in MSH induced ocular hypotension. MSH and its analogues might have clinical relevance as antiglaucoma drugs with fewer side-effects because of their antiallergic and anti-inflammatory properties.
Dr. N. Naveh, Goldschleger Eye Research Institute, Tel Aviv University School of Medicine, Sheba Medical Center, Tel Aviv, Israel
Classification:
5 Experimental glaucoma; animal models
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
