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Abstract #6006 Published in IGR 2-2
Inner nuclear layer cell loss in rabbits with experimental glaucoma
Song Z; Cui S; Zhang DChinese Ophthalmic Research 2000; 18: 126-127
OBJECTIVE: To observe the changes of inner nuclear layer (INL) cells in rabbit retinas with experimental glaucoma. METHODS: Ten Chinese rabbits were used in the research. 2.5% methylcellulose 0.2 ml was injected into one eye of each rabbit to elevate intraocular pressure (IOP), and the other eye of the rabbit received 0.2 ml normal saline as a control. The injection was repeated once a week. For histology studies, the rabbits were killed and the eyes were enucleated at the end of four weeks. Light and electron microscopy were used to study the retina. With an ocular frame, ganglions (ten lattice) and INL (one lattice) cells were counted by light microscopy. The pair test was performed to analyze the result of cell numbers. RESULTS: There were karyopyknosis, chromatin and nuclear membrane rupture in the ganglion cells, and some INT cells had marked lytic changes. The numbers of ganglion and INL cells had decreased. In control eyes, ganglion cells were (17.90 ± 2.33)/10 L, and INL cells were 14.50 ± 2.12 L; while, in experimental eyes, ganglion cells were 8.00 ± 1.63)/10 L and INL cells were 11.10 ± 1.79 L. The numbers of ganglions and INL cells were significantly different between the two groups. CONCLUSIONS: These results showed that INL cells degeneration and death occurred in rabbit retinas after elevated IOP, which suggests that INL cells may be lost in some glaucoma conditions. LA: Chinese
Dr. Z. Song, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, China
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.2 Electron microscopy (Part of: 3 Laboratory methods)
5 Experimental glaucoma; animal models
