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Abstract #6366 Published in IGR 3-2
A study on susceptibility of different layers of rabbit retina to ocular hypertension
Luo R; Ge J; Lin JChinese Journal of Ophthalmology 2001; 37: 302
OBJECTIVE: To investigate the histological damage from ocular hypertension and its degrees in different layers of the rabbit retina. METHODS: Intraocular pressure (IOP) was elevated by intracameral injection of 0.10-0.15 ml 2% methylcellulose in 16 gray rabbits. The rabbit eyes were enucleated, fixed, and paraffin-embedded. For histological evaluation, the slides were stained with hematoxylin-eosin, and examined by light microscopic and computer automatic image analysis. RESULTS: Quantitative analysis indicated that all the retinal layers have different degrees of histopathological damage. Among these, the retinal ganglion cells (RGCs) and retinal nerve fiber layer (RNFL) were the most severely damaged, with decrease rates of 74.3% and 61.9%, respectively. The decrease rates of the other retinal layers were as follows: inner retinal layer, 42.4%; whole sensory layer of the retina, 35.0%; outer layer, 24.2%. The authors also found a significant correlation between RGC loss and RNFL thinning (r = 0.68, p < 0.01), demonstrating a strong correlation between IOP level and RNFL thickness (F = 8.97, p < 0.01). CONCLUSION: All the anatomical layers of the retina can suffer from different degrees of degeneration and atrophy.LA: Chinese
Dr R. Luo, Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China
Classification:
5 Experimental glaucoma; animal models
3.1 Microscopy (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
