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Abstract #3699 Published in IGR 4-2
Effect of pressure on proliferation and apoptosis of bovine trabecular meshwork
Xue W; Du S; Li YChinese Journal of Ophthalmology 2002; 38: 340-343
OBJECTIVE: To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl-2 and bax mRNA by bovine trabecular meshwork cells. METHODS: Twenty, 40, 60, and 80 mmHg (1 mmHg = 0.133 kPa) pressure were given to the cultured trabecular meshwork cells, respectively, in the treatment groups. No pressure was given in the control groups. Each pressure sustained for 24 and 48 hours. Proliferation index, apoptosis index, bcl-2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, flow cytometry and in situ hybridization. RESULTS: In the 24-hour groups, there were no significant differences between the apoptosis index of control group and pressure ≤ 40 mmHg groups. But in the 40 mmHg groups, the proliferative index decreased and bax mRNA expression increased significantly. The apoptosis index increased remarkably when the pressure was ≥ 60 mmHg. In the 48-hour groups, apoptosis index, proliferative index and bax mRNA expression was significantly different from that of the control groups when pressure was ≥ 40 mmHg. CONCLUSIONS: Pressure ≥ 40 mmHg sustained for a period of time can suppress trabecular meshwork cell proliferation and induce apoptosis through influencing bcl-2 family members. LA: Chinese
Dr. W. Xue, Department of Ophthalmology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xuewei@public.wuhan.cngb.com
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
4 Tissue culture of ocular cells
