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Abstract #14238 Published in IGR 8-3
Induction of heat shock protein 27 in retinal ganglion cells and its role in a rat glaucoma model
Lu H-B; Yuan Y-S; Luo Q-L; Li Y; Liu QInternational Journal of Ophthalmology 2006; 6: 264-270
AIM: To investigate whether heat shock protein 27 (HSP27) is induced in retinal ganglion cells (RGCs) in experimental rat glaucoma and whether the induction of HSP27 by intraocular pressure (IOP) elevation can increase serum autoantibody to HSP27 in the model. METHODS: 55 Wistar rats were randomly divided into IOP elevation, Sham and normal groups by SPSS12.0. IOP was raised by electrocoagulating at least 3 episcleral veins and limbal veins on the right eye of each rat in IOP elevation group and its contralateral eye was used as controls. Immunohistochemical staining for HSP27 was performed in RGCs and retinal nerve fiber layer (RNFL) and serum immunoreactivity against HSP27 was detected by means of enzyme-linked immunosorbent assay (ELISA) in three groups. RESULTS: HSP27 expression increased in RGCs and RN FL of the eyes with IOP elevation, while it was expressed weakly in untreated control eyes. Compared with sham and normal groups, serum autoantibody to HSP27 was slightly high at 1 week (P > 0.05) and significantly increased at 2, 3, 4 and 8 weeks (P < 0.05) in IOP elevation group. CONCLUSION: These results demonstrate the enhanced expression of the endogenous HSP27 might play an important role in glaucomatous optic neuropathy.
Dr. H.-B. Lu, Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041 Sichuan Province, China
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)