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Abstract #21510 Published in IGR 10-3

Establishment of chronic glaucoma model and changes of free glutamate concentration in vitreous

Liu D; Chen X; Pan R; Li M
Chinese Ophthalmic Research 2008; 26: 343-346


OBJECTIVE: Glutamate is thought to be responsible for the death of retinal ganglion cells that are characteristics of glaucoma. The purpose of this paper was to investigate the damage of retinal optic nerve in chronic ocular hypertension rat and the changes of free glutamate concentration in vitreous humor. METHODS: Experimental glaucoma was induced unilaterally in the right eyes of 30 health adult Wister rats using a Krypton laser with wavelength of 532 nm aimed at the trabecular meshwork, and the left eyes were as control. Intraocular pressure (IOP) was measured using the Tono-Pen before and immediately after surgery and then at one week interval. 1% toluidine blue staining of whole mounted retina was utilized to label retinal nerve cells, and these cells were counted in the 3rd, 6th and 9th week respectively. Rat models were sacrificed in the 1st, 2nd and 3rd group rats. The free glutamate concentration in vitreous humor was measured by High-Performance Liquid Chromatographic Analysis (HPLCA). RESULTS: IOP of the experimental eyes was higher than that of the control eyes. The density value of the retinal ganglion cells in the experimental eyes was 2 124 ± 108/mm2, 1 865 ± 136/mm2 and 1 654 ± 125/mm2 at the 3rd, 6th and 9th week respectively, and the value of control eyes was 2 516 ± 196/mm2, exhibiting a significant difference among the experimental eyes and the control eyes (F = 5.496, P = 0.037). The free glutamate concentration in vitreous humor was 20.10 ± 2.45 μmol/L, 22.35 ± 2.75 μmol/L and 23.56 ± 2.80 μmol/L at the 3rd, 6th and 9th week in the experimental eyes, and 18.32 ± 2.3 μmol/L in the control eyes without significant difference among the glaucoma eyes and the control eyes (F = 0.796, P = 0.661). CONCLUSION: IOP presents a chronical and moderate increase in an injury eye of trabecular meshwork by laser. The density of retinal nerve cells appear to be a decent tendency and the free glutamate concentration in vitreous humor is gentally increased in this experimental glaucoma model. LA: Chinese

Dr. D. Liu, Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China. ldj1213@163.com


Classification:

5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.7 Biochemistry (Part of: 3 Laboratory methods)
2.11 Vitreous body (Part of: 2 Anatomical structures in glaucoma)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)



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