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Abstract #21619 Published in IGR 10-3
Expression of caspase-9 affected by AG on retina of rats with chronic IOP elevation
Nie Q-Z; Sha Q; Wang Y-S; Gui D-M; Liu Z-L; Gao D-WInternational Journal of Ophthalmology 2008; 8: 1299-1303
AIM: To study caspase-9 expression on rat retina in the process of chronic elevation of IOP and the changes with the application of amino guanidine (AG), thus to investigate potential protective function of AG to rat retina with chronic elevation of IOP. METHODS: Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and expression of caspase-9 at different time points of rat with chronic IOP elevation, both affected or not affected by the application of AG. RESULTS: Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21st day of chronic IOP elevation, retinas became thinner and the quantity of retinal ganglion cells (RGCs) decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9. CONCLUSION: Apoptosis-related gene caspase-9 played a part in the process of chronic IOP elevation; AG protects retina by down-regulating expression of caspase-9.
Dr. Q. Sha, Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang 110004 Liaoning Province, China. nieyjin@126.com
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)