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Abstract #21647 Published in IGR 10-3
Expression of connective tissue growth factor after trabeculectomy in rabbit ocular model
Tang W-H; Wang C-Y; Ke M-K; Ou-Yang J; Liu Y-WInternational Journal of Ophthalmology 2008; 8: 896-898
AIM: To evaluate the role of connective tissue growth factor (CTGF) in scar formation of the filtering bleb that occurs after trabeculectomy by observing the expression of CTGF in the scarring process of the filtering bleb after surgery in a rabbit model. METHODS: Trabeculectomy was randomly performed on one of eyes of 28 healthy adult rabbits, and the other eye was used as the control. On postoperative day 1, 3, 5, 7, 10, 14 and 21, the rabbits were killed in batch. Two other rabbits without trabeculectomy were killed before surgery as normal controls. Tissues were harvested from the bleb area and made into pathological section. After HE staining and immunohistochemical staining, the sections were observed under light microscope. Protein expression was quantitatively analyzed by image analysis technique. RESULTS: There was nearly no CTGF expression in the normal control tissue. The expression of CTGF was significantly upregulated and peaked prominently at postoperative 5th day, mainly in the fibroblast cytoplasm. CONCLUSION: CTGF plays an important role in the scarring process of the filtering bleb after trabeculectomy. Inhibition of CTGF synthesis or its activation may help to prevent the failure of glaucoma filtering surgery. LA: Chinese
Dr. W.-H. Tang, Department of Ophthalmology, First People's Hospital of Jiujiang City, Jiujiang 332000 Jiangxi Province, China. twh120@yahoo.com.cn
Classification:
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)