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Abstract #21698 Published in IGR 10-3
Endothelin-1 (ET-1) is increased in rat retina after crushing optic nerve
Oku H; Fukuhara M; Kurimoto T; Okuno T; Sugiyama T; Ikeda TCurrent Eye Research 2008; 33: 611-620
PURPOSE: To determine the alterations in the expression of endothelin-1 (ET-1) and its specific receptors in the sensory retina after optic nerve injury. METHODS: The optic nerve of the right eye of Wistar rats was crushed. The sensory retinas were removed 1, 2, 4, 7, and 14 days after the surgery, and the amount of ET-1 in the retinas was measured by radioimmunoassay, and the mRNA levels of ETA and ETB receptors were determined by real-time PCR. RESULTS: The ET-1 levels in the sensory retinas following the optic nerve crush were 8.83 ± 2.23 and 9.99 ± 4.83 pg/mg protein on days 7 and 14 after the injury, while those in the sham controls were 4.55 ± 1.36 and 4.85 ± 1.57 pg/mg protein, respectively. The increase was significant on day 7 (p = 0.003, ANOVA followed by t- test), but not on day 14 (p = 0.054). The mRNA levels of the ETA and ETB receptors in the retina after the optic nerve crush was significantly increased on day 7. A two-fold increase was recognized in ETB receptors. Immunohistochemical analyses revealed that the ET-1 expression was increased mainly in the inner retinal layers, including the nerve fiber layer and ganglion cell layer (GCL). The increased immunoreactivity of the ETB receptor was seen in the GCL cells and also in the outer nuclear layer. CONCLUSION: An increase in the intraretinal ET-1 may be involved in the retinal remodeling after the optic nerve is crushed.
Dr. H. Oku, Department of Ophthalmology, Osaka Medical College, Osaka, Japan. hidehirooku@aol.com
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
