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Abstract #19345 Published in IGR 9-3
Expression of glutamine synthetase mRNA in rat retina following acute intraocular hypertension
Zhang J; Wang J; Wang D; Bai H; Kang JChinese Ophthalmic Research 2007; 25: 373-375
OBJECTIVE: Retinal ischemia/reperfusion induce the necrosis and apoptosis of retinal ganglion cells (RGCs) , which release amount of glutathione and therefore aggravate the damage of RGCs. This study was curried out to observe the expression change of glutamine synthetase(GS) mRNA in the glaucomatous retina. METHODS: Acute intraocular hypertention was induced by increasing anterior chamber pressure to 110 mmHg for 60 min in the left eyes of 30 Wistar rats using elevating the perfusion container to 150 cm, and anterior chamber incision alone was performed in other 5 rats. The right eyes of all the rats were as controls. Total RNA in retina was extracted and GSmRNA was semiquantitated by way of RT-PCR at 4, 12 , 24, 36, 72 and 120 hours after removal of container. RNA purity was calculated at OD260 nm value. RESULTS: Expression level of GSmRNA was recorded as GSmRNA/GAPDHmRNA. In comparison with control group , the expression of GSmRNA was increased 4 hours after acute intraocular hypertentionf (1. 100 ± 0.122), peaked at 24 hours (1.511 ± 0.125), and descended at 36 hours (1. 282 ± 0.131), showing a statistically significant difference in comparison with the control group (0. 852 ± 0.115). The expression of GSmRNA reverted to (0. 953 ± 0.104) 72 hours after acute intraocular hypertention without significant difference compared with the control group. CONCLUSION: The GSmRNA is overexpressed in acute hypertentive retina, which possibly results in the upregulation of GS that transforms glutamate into glutamine and ultimately mitigates the damage of retinal ganglion cells. LA: Chinese
Dr. J. Zhang, Qingdao University, Qingdao 266003, China. junfuzhang2004@sina.com
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
